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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: J Immunol. 2010 Mar 26;184(9):5141–5150. doi: 10.4049/jimmunol.0903413

FIGURE 4.

FIGURE 4

Colocalization of the F. novicida WT strain and mutant strains with p47phox in neutrophils. A, Colocalization of the F. novicida WT and ΔABCH or ΔacpA mutant strains with p47phox was determined at 5, 15, and 30 min postinfection in neutrophils. Francisella was detected following staining with goat anti-mouse Alexa Fluor 488 (green color), and p47phox was detected following staining with donkey anti-rabbit Alexa Fluor 546 (red color). Representative confocal microscopy images of F. novicida and ΔABCH colocalized with p47phox within neutrophils are shown at 30 min postinfection. The images are representative of 1000 infected cells examined from triplicate coverslips in three independent experiments. Original magnification ×63. B, Colocalization of the F. novicida WT, ΔacpA, and ΔABCH mutant strains with p47phox was quantified at 5, 15, and 30 min postinfection. Analyses were based on examination of 1000 infected cells examined from triplicate coverslips in three independent experiments. The results shown are cumulative data of three experiments (mean ± SD of nine samples; triplicate samples were included in each test group in each experiment).