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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: J Immunol. 2010 Mar 26;184(9):5141–5150. doi: 10.4049/jimmunol.0903413

FIGURE 6.

FIGURE 6

The effect of F. novicida WT and acp mutants on phosphorylation of p47phox and p40phox in neutrophils. Neutrophils were incubated with F. novicida WT or ΔABCH strains for the times shown. Lysates were loaded by protein equivalents, separated by SDS-PAGE, and analyzed by Western blotting with Abs specific for phosphorylated p47phox (rabbit anti-Pp47phox) and phosphorylated p40phox (rabbit anti-Pp40phox). The same membrane was reprobed with β-actin Ab to verify equal protein loading. Resting (R) represents the uninfected PMN cell lysates collected at 30 min postinfection. A representative Western blot image is shown (n = 7).