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. 2010 Sep 21;2011:248592. doi: 10.1155/2011/248592

Figure 4.

Figure 4

Suppressive effect of CME on the production of LPS-induced proinflammatory cytokines in RAW 264.7 cells. The RAW 264.7 cells were pretreated with CME (5, 10, 20, and 50 μg/mL) for 1 hour, and then stimulated with LPS (1 μg/mL). After 6 hours, the total mRNA was isolated, and the mRNA levels of (a) TNF-α and (b) IL-6 were examined by RT-PCR. * indicates an increase in mRNA expression relative to the control (P < .05), ** indicates a decrease in mRNA expression relative to the LPS group (P < .05).