Skip to main content
. Author manuscript; available in PMC: 2011 Sep 24.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Aug 17;400(3):299–304. doi: 10.1016/j.bbrc.2010.08.043

Fig. 4.

Fig. 4

sFRP2 enhances Wnt3a-mediated transcriptional activation in HSG cells (A) but not in L-cells (B). Cells were co-transfected with TopFlash (□) or FopFlash (■) construct and Renilla (transfection control) plasmids and 24 h later treated with Wnt3a alone or together with indicated sFRP2 for 24h. (C) After transfection with TopFlash and Renilla plasmids, HEK293A cells were treated with L-cell-conditioned medium plus/minus 3 nM Wnt3a. For inhibition experiments, cells were pretreated with 6 nM DKK1 for 30 minutes. For controls, cells were treated with Wnt3a and sFRP2 or with 50 % CM alone. After 24 h, cell lysates were analyzed for luciferase activity. Shown are mean ± SEM of at least three experiments, each performed in triplicate wells. ***P<0.0001; **P<0.001; *P<0.01 compared with control by using of ANOVA and the Newman-Keuls test.