Figure 3.

Transfection efficiency of minicircle in vitro and in vivo. (A) HL-1 cells were transfected with MC-Fluc-eGFP (MC-LG) in 6-well plate. (B) A robust correlation exists between minicircle dosage and bioluminescence signals (r²=0.96). Each data point is from an individual observation. Pearson’s correlation was used. (C) Bioluminescence imaging and (D) quantitative analysis indicate minicircle plasmid-mediated gene expression was stable for at least 8 weeks in the heart compared to <4 weeks using regular plasmid (data not shown).