Table I. TLR and reference gene primer sequences.

Symbol	Accession a	Gene name	Sequences (5′ - 3′) b	L c
GAPDH	NM_002046	Glyceraldehyde-3-phosphate dehydrogenase	F: AAGGTCGGAGTCAACGGATTT R: ACCAGAGTTAAAAGCAGCCCTG	66
RPLP0	NM_001002	Large ribosomal protein P0	F: CCTCATATCCGGGGGAATGTG R: GCAGCAGCTGGCACCTTATTG	95
TLR1	NM_003263.3	Human toll-like receptor 1	F: AGGCCTTGTCTATACACCAAGTTGT R: CGTGTACCAGACACTGTGAAATTTT	112
TLR2	NM_003264.3	Human toll-like receptor 2	F: AACCGGAGAGACTTTGCTCA R: CCACTGACAAGTTTCAGG CA	91
TLR3	NM_003265.2	Human toll-like receptor 3	F: CCTGGTTTGTTAATTGGATTAACGA R: TGAGGTGGAGTGTTGCAAAGG	82
TLR4	NM_138554.2	Human toll-like receptor 4	F: GGACTGGGTAAGGAATGAGCTAGTA R: CACACCGGGAATAAAGTCTCTGT	94
TLR6	NM_006068.2	Human toll-like receptor 6	F: TGTACACCGTGTTTTCTGAGATGA R: GAAAACGTTCTGGGTAAAGTTCAAA	125
TLR7	NM_016562.3	Human toll-like receptor 7	F: AAGCCCTTTCAGAAGTCCAAGTT R: GGTGAGCTTGCGGGTTTGT	91
TLR8	NM_016610.2	Human toll-like receptor 8	F: GCTGCTGCA AGTTAC GGAAT R: CGC ATAACTCACAGGAACCA	118
TLR9	NM_017442.2	Human toll-like receptor 9	F: TGAAGACTTCAGGCCCAACTG R: TGCACGGTCACCAGGTTGT	75

^aGenBank accession number.

^bSources for primer sequences were RTPrimerDB (http://medgen.ugent.be/rtprimerdb/, GAPDH and RPLP0) ²¹, qPrimerDepot (http://primerdepot.nci.nih.gov/, TLR2 and TLR8) ²⁰, and Rebbapragada et al. (TLR1, TLR3, TLR4, TLR6, TLR7, and TLR9) ²².

^cAmplicon length (bp)