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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Exp Gerontol. 2010 Jun 23;45(11):825–833. doi: 10.1016/j.exger.2010.06.002

Figure 3. Northern analysis of conditional transgene expression in adult flies.

Figure 3

The indicated transgenic strains containing either the RNAi-DmMRG15 construct, the DmMRG15 wild type construct, or the MT1 construct were crossed to the rtTA(3)E2 driver strain or to the Act-GS-255B driver strain, as indicated. Progeny containing both constructs were cultured in the presence and absence of drug for one week. The drug was doxycycline for rtTA(3)E2 crosses and RU486 for Act-GS-255B crosses. Control flies were the progeny of a cross of the drivers to Oregon-R wild-type flies or to strain bearing a PGM-RNAi construct, as indicated. Total RNA was analyzed by Northern blot, and the membrane was hybridized sequentially to probes that will recognize the endogenous DmMRG15 mRNA and the Rp49 loading control, as indicated. Data for males is presented in the upper two panels and data for females in the lower two panels. Band intensities were quantified, and DmMRG15 expression was normalized to the Rp49 loading control. The percent change in DmMRG15 message levels between minus-drug and plus-drug samples is indicated below each pair of lanes. Values in parentheses indicates uncertainty in measurements due to interference from larger transcripts associated with line DmMRG-WT8.