Figure 4. Life span analyses using the Tet-on doxycycline-regulated system.
The indicated transgenic strains were crossed to the rtTA(3)E2 driver strain, and the adult progeny containing both constructs were assayed for survival when cultured on food plus and minus doxycycline. Control flies were generated by a cross of Oregon-R wild type strain to rtTA(3)E2. The percent change in life span and p value from log-rank test are indicated in each panel. Additional statistical details and results of replicate experiments are summarized in Table 2. The plotted data are from replicate experiment number 2 (Table 2). A. Control flies. B. Line RNAi-DmMRG15-4-1. C. Line RNAi-DmMRG15–53. D. Line RNAi-MRG15-4-3.