Figure 2. ANG-II evokes InsP₃-dependent Ca²⁺ release in cardiomyocytes.

Ca²⁺ release from internal stores was monitored using the low-affinity Ca²⁺-indicator Mag-Fluo-4/AM. (a, b) Representative traces. (a) ANG-II causes Ca²⁺ release that recovers to baseline. (b) No significant Ca²⁺ release was observed in cardiomyocytes pre-treated with either one of the InsP₃R inhibitors 2-APB or XeD. (c) Quantification of released Ca²⁺. ANG-II causes Ca²⁺ release only in the absence of InsP₃R inhibitors. (d) Representative pseudo-colored time course of cardiomyocytes challenged with ANG-II. Letters indicate respective time points in panel a. Scale bar: 10 μm.