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. Author manuscript; available in PMC: 2010 Oct 11.
Published in final edited form as: J Biol Chem. 2003 Dec 29;279(12):10939–10945. doi: 10.1074/jbc.M310291200

Fig.5. Specificity of α1(XI)Npp interaction by co-precipitation of endogenous α1(XI)Npp with recombinant α1(XI)Npp-nickel-agarose.

Fig.5

Proteins were extracted from chondrocyte pellet cultures in 1 m NaCl-containing buffer. Buffer conditions were changed by dialysis to reduce the salt concentration to 150 mm NaCl, and extract was incubated with α1(XI)Npp-bound nickel-agarose beads. Proteins were resolved by SDS-PAGE 12% polyacrylamide gel and analyzed by immunoblot using an antibody to α1(XI)Npp and an antibody to α2(XI)Npp. Lanes 1 and 3, material not bound to α1(XI)Npp-bound nickel-agarose beads. Lanes 2 and 4, material bound to α1(XI)Npp-bound nickel-agarose beads. Lanes 1 and 2, immunoblot with α2(XI)Npp antibody. Lanes 3 and 4, immunoblot with α1(XI)Npp antibody. Lane 5, molecular weight markers. Arrows on left-hand side indicate position of the α1(XI)Npp and the α2(XI)Npp.