. Author manuscript; available in PMC: 2010 Oct 11.

Published in final edited form as: J Immunol. 2010 Apr 5;184(9):5280–5286. doi: 10.4049/jimmunol.0903282

Table II.

Profiles of polyunsaturated n-6 and n-3 fatty acids in serum of (NZB × NZW)F1 mice fed with specialized corn oil (CO) and fish oil (FO) diets enriched with eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA).

Fatty acids	CO	FO-18/12	FO-EPA	FO-DHA
18:2n-6	30.33±0.70^a	13.69±0.31^b	9.79±0.49^c	8.85±0.37^c
20:3n-6	0.28±0.03	0.27±0.00	nd	nd
20:4n-6	19.52±0.36^a	9.59±0.25^c	12.51±0.48^b	5.21±0.22^d
20:5n-3	nd	20.17±1.33	22.69±1.17	20.76±0.41
22:5n-6	0.34±0.12	nd	nd	nd
22:5n-3	nd	0.39±0.02^a	0.96±0.15^b	0.70±0.07^b
22:6n-3	3.33±0.40^a	12.55±0.28^c	9.89±0.49^b	23.89±0.74^d

PUFA	53.79±0.74	56.48±0.56	55.84±0.92	59.40±0.62
n-3 FA	3.33±0.40^a	33.11±1.20^b	33.54±1.01^b	45.34±0.66^c
n-6 FA	50.46±0.48^a	23.37±0.64^b	22.30±0.24^b	14.06±0.57^c
n-6/n-3	15.85±1.90	0.71±0.05	0.67±0.03	0.31±0.02

CO, corn oil; DHA, docosahexaenoic acid; EPA, eicosapentaenoic acid; FA, fatty acids; FO, fish oil; nd – not detected; PUFA, polyunsaturated fatty acids. Total lipids of (NZB × NZW)F1 mice serum were extracted, methylated and subjected to analysis by gas chromatography. The values (% of total fatty acids) are means of three independent measurements ± SEM. n=5. Significant difference (P<0.05) is indicated with different alphabets analyzed by ANOVA followed by Newman-Keuls test. Ratio of n-6/n-3 fatty acids is expressed as (18:2n-6 + 20:3n-6 +20:4n-6 +22:5n-6)/(20:5n-3 + 22:5n-3 + 22:6n-3).