Table III.
Proteinuria of (NZB × NZW)F1 mice fed with specialized corn oil (CO) and fish oil (FO) diets enriched with eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA).
| Groups (diet) | Age (Months) | Number of mice | Proteinuria Levels1 | |||
|---|---|---|---|---|---|---|
| Trace (<30 mg/dl) | + (30–100 mg/dl) | ++ (100–500 mg/dl) | +++ (>500 mg/dl) | |||
| CO | 4 | 15 | 3 | 0 | 0 | 0 |
| 6 | 13 | 7 | 2 | 1 | 0 | |
| 8 | 10 | 3 | 0 | 7 | 0 | |
| 10 | 5 | 0 | 0 | 4 | 1 | |
| 12 | 4 | 0 | 0 | 1 | 3 | |
| FO-18/12 | 4 | 15 | 4 | 0 | 0 | 0 |
| 6 | 15 | 4 | 1 | 0 | 0 | |
| 8* | 13 | 5 | 2 | 0 | 0 | |
| 10* | 11 | 6 | 1 | 1 | 0 | |
| 12 | 8 | 0 | 6 | 2 | 0 | |
| FO-EPA | 4 | 15 | 2 | 0 | 0 | 0 |
| 6 | 12 | 4 | 2 | 0 | 0 | |
| 8* | 7 | 5 | 1 | 1 | 0 | |
| 10* | 6 | 0 | 4 | 1 | 1 | |
| 12 | 6 | 0 | 1 | 2 | 2 | |
| FO-DHA | 4 | 15 | 1 | 0 | 0 | 0 |
| 6 | 14 | 2 | 0 | 0 | 0 | |
| 8 | 14 | 2 | 0 | 0 | 0 | |
| 10*† | 13 | 1 | 1 | 0 | 0 | |
| 12*† | 11 | 1 | 1 | 1 | 0 | |
At 2-months of age, mice were switched to semi-purified diets containing 10% corn oil (control), and fish oils (FO) enriched in eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA): 1) 18/12 fish oil (FO-18/12) 2) 55/5 EPA-enriched FO (FO-EPA), and 3) 5/60 DHA-enriched FO (FO-DHA).
1
Proteinuria was determined using Chemstrips (Roche Diagnostic Corporation, Indianapolis, Inc.) (n=15).
Different signs (* and *†) indicates significant difference in proteinuria levels of FO-18/12, FO-EPA, FO-DHA fed mice compared to CO fed mice measured by ANOVA.