Fig. 7.
hrGFP expression was not influenced by sodium butyrate treatment during differentiation. BGO1V2-EFG cells were incubated with sodium butyrate (0.02, 0.2, and 2 mM) for 48 hr at an early stage and at a later stage of cell differentiation as described in Methods. The effect of sodium butyrate on hrGFP expression was evaluated by counting hrGFP+ cells (green) vs total cells (DAPI, blue) at the early stage (A) or at the later stage of differentiation (B). hrGFP expression was not influenced by sodium butyrate treatment at either the early stage (C) or the later stage (D) of differentiation. Scale bar = 50 μm.