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. Author manuscript; available in PMC: 2011 Sep 8.
Published in final edited form as: J Am Chem Soc. 2010 Sep 8;132(35):12286–12298. doi: 10.1021/ja910342d

Figure 2.

Figure 2

Reaction coordinate diagram of Af2Sir2 catalyzed reactions. The irreversible step is nicotinamide-glycosidic bond breaking and making (k3 and k4). A. At saturating nicotinamide concentration, the maximum reverse commitment was found to be 0.955. B. The relevant reaction coordinate during KIE measurements. Under these conditions the average nicotinamide concentration is 40 μM, and the reverse commitment during experiment is 0.038.