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. Author manuscript; available in PMC: 2011 Jan 15.
Published in final edited form as: Cancer Res. 2010 Jan 12;70(2):532–542. doi: 10.1158/0008-5472.CAN-09-1477

Figure 3. BRCA1 and BARD1 negatively regulate transcription from the BRCA1 promoter.

Figure 3

(A) Jurkat T cells were cotransfected with luciferase reporter constructs (see schematic above) spanning the indicated lengths of regulatory sequences upstream of the BRCA1 TSS (pGL12 or pGL35) in combination with either: BRCA1 wt (pcDNA3 BRCA1), BRCA1 C-terminal mutant (pcDNA3 ΔBRCT) or empty (pcDNA3) expression vectors. (B) Jurkat T-cells were cotransfected with the indicated BRCA1 promoter reporters and either shRNA BRCA1 or control shRNA with scrambled sequence expression vectors. (C) Jurkat T-cells were cotransfected with the indicated BRCA1 promoter reporters and either wild type BARD1 (BARD1) or empty expression vectors or BARD1 siRNA or control duplex siRNA oligonucleotides. Error bars show the standard error of the mean from at least 3 independent transfections. (D) ChIP analysis of BARD1 enrichment at the BRCA1 promoter with and without pretreatment with 1 µM doxorubicin. Error bars indicate the standard error of the mean derived from 2 independent biological replicates.