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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Hum Immunol. 2010 Jun 20;71(10):1018–1026. doi: 10.1016/j.humimm.2010.05.019

Figure 3. TpsnΔEx3 interacts with TAP.

Figure 3

M553 cells were treated with IFN for 16-20 hours and metabolically labeled using 35S methionine prior to lysis in digitonin containing lysis buffer. Cell lysates were subjected to immunoprecipitation (1°IP) using a TAP2 specific antibody. After elution, re-IP (2°IP) with a control antibody (Ctrl) or TO-3 (Tpsn) was performed and gels subject to autoradiography.