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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Hum Immunol. 2010 Jun 20;71(10):1018–1026. doi: 10.1016/j.humimm.2010.05.019

Figure 4. TpsnΔN50 downregulates surface MHC class I molecules by competing with wild type tapasin in HeLa cells.

Figure 4

A. The indicated puromycin resistant transfectants of HeLaM cells were labeled with 35S-methionine and immunoprecipitated for tpsnΔN50 (Rgp48C) or calreticulin. B. HLA-A (CR11.351) and HLA-B (SFR8.B6) expression on the surface of clones indicated. Mean Fluorescence Intensity (MFI) is plotted for each clone. C. Clones F7 (expressing tpsnN50) and E7 (not expressing tpsnN50), were lysed in digitonin and immunoprecipitated using a Ring4C (TAP1) specific antibody. After elution, re-immnoprecipitation was performed using control, heavy chain (HC, 3B10.7), tapasin (R.gp48C) or β2m specific antibodies. A representative figure of at least three independent experiments is shown.