Figure 7. YFP-N300 downregulates MHC class I expression in M553(R240)tpsn cells.
M553(R240)Tpsn cells were transiently transfected with YFP-N300 or co-transfected with empty vector and pmaxGFP. Twenty-four hours post transfection, MHC class I expression was analyzed by flow cytometry using a PE directly conjugated W6/32 (MHC class I) antibody. Mean fluorescent intensity was determined for W6/32 staining on populations of cells gated on forward and side scatter (R1) and for the negative or positive expression of GFP/YFP (R2, GFP/YFP negative) and R3, (GFP/YFP positive).