Table 3. Effects of C2Cer Treatment on Brain Neuronal and Oligodendrocyte Protein Expression.

Long Evans rat pups were treated with ceramide analogs, D-erythro-N-Acetyl-Sphingosine (C2Cer-bioactive) or D-erythro-Dihydro-N-Acetyl-Sphingosine (C2 Dihydroceramide; C2DCer-inactive) by i.p. injection (see Methods). Protein homogenates prepared in NP-40 lysis buffer were used to measure immunoreactivity in direct binding ELISAs with results normalized to ribonuclear protein level in each well. Results represent the mean ± S.E.M. of the calculated ratios of immunoreactivity. Inter-group statistical comparisons were made using Student T-tests and significant p-values are indicated

	Temporal lobe			Cerebellum
Protein	C2Cer	C2DCer	p-value	C2Cer	C2DCer	p-value
Hu	12.53 ± 1.01	17.19 ± 0.95	0.0015	20.68 ± 0.43	19.98 ± 0.71
MAG-1	23.20 ± 0.98	22.07 ± 1.33		15.46 ± 0.70	14.15 ± 0.57
pTau	25.38 ± 1.25	27.76 ± 1.67		26.14 ± 1.59	19.86 ± 0.74	0.0005
Tau	410.10 ± 16.60	411.00 ± 15.89		342.90 ± 12.73	306.50 ± 9.90	0.02