Figure 2.

Susceptibility of various breast cancer cells to Ad5/3, delta24-pIX-mCherry virus infection in vitro. A panel of breast cancer cell lines were infected with Ad5/3, delta24-pIX-mCherry (mCherry-CRAd) particles at a multiplicity of infection of 1 TCID₅₀/cell, and fluorescence was visualized 48 hours later by fluorescence microscopy at different magnifications using 4× or 20× objectives. Bright field and fluorescent images of the same views were taken in parallel to assess the early cytopathic effect of the replicating CRAd. Normal human fibroblasts VH-10 and lung carcinoma A549 cells were used as negative and positive controls for the cancer-selective virus replication, respectively. DY36T2 and MDA-MB-361 cell lines (highlighted in red) were chosen for implantation in mice to generate tumor xenografts. The asterisk indicates that the origin of the MDA-MB-435 cell line is highly controversial and was recently suggested to be a melanoma rather than a breast cancer line. However, the controversy has not been fully resolved.²⁵