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. Author manuscript; available in PMC: 2011 Sep 14.
Published in final edited form as: Biochemistry. 2010 Sep 14;49(36):7821–7829. doi: 10.1021/bi100726n

Figure 3.

Figure 3

TRUSS interacts with TRAF2 via amino acids 249 to 440. A) TRAF2 co-immunoprecipitation of TRUSS deletion mutants. HEK293 cells, transfected with 1 μg of each HA-tagged TRUSS deletion mutant construct plus 0.5 μg Flag-tagged TRAF2 construct, were lysed and immunoprecipitated with anti-TRAF2 antibody or non-immune IgG as a control. Western blots were probed with anti-HA antibody and then with anti-TRAF2. Error bars in panel A are the mean ± SEM calculated from between 3 and 6 independent experiments. The number of experiments for each construct is shown in parentheses. B) TRAF2 co-immunoprecipitates with TRUSS249-440. HEK293 cells were transfected with 1 μg of TRUSS249-440 plus 0.5 μg Flag-tagged TRAF2 construct, lysed, and lysates immunoprecipitated with anti-TRAF2 antibody or non-immume IgG. Western blots were probed with an anti-HA antibody then with anti-TRAF2. Representative blot of 4 independent experiments.