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Mass spectrometry analysis of ceramide species in HAECs. HAECs were incubated overnight with or without with 0.1 µM HN followed by an incubation with 100 µg/mL Ox-LDL for 6 h. Cells were then harvested and ceramide species were analysed by mass spectrometry (HPLC/MS/MS) from lipid extracts of the cells (see Section 2).
