Table 1.
Cardiovascular defects are observed in Tc1 but not Ts1Rhr embryos
| Stage | Paternal background | Heart defects |
Normal hearts | % heart defects | |||
|---|---|---|---|---|---|---|---|
| Genotype | VS | AVC | OFT | ||||
| E14.5 | 129S8:C57BL/6J (F1) | Wild-type (n = 30) | 3 | 0 | 0 | 27 | 10.0 |
| Tc1 (n = 29) | 11 | 1 | 5 | 18 | 37.9§ | ||
| E14.5 | C57BL/6J | Wild-type (n = 51) | 7 | 0 | 1 | 44 | 13.7 |
| Tc1 (n = 49) | 26 | 15 | 12 | 22 | 55.1* | ||
| E18.5 | C57BL/6J | Wild-type (n = 22) | 0 | 0 | 0 | 22 | 0.0 |
| Tc1 (n = 33) | 7 | 1 | 1 | 26 | 21.2# | ||
| E14.5 | Wild-type (n = 22) | 0 | 0 | 0 | 22 | 0.0 | |
| Ts1Rhr (n = 19) | 1 | 0 | 0 | 18 | 5.3 | ||
A spectrum of heart malformations is observed in Tc1 hearts at E14.5 and E18.5, affecting the ventricular septum (VS), atrioventricular canal (AVC), and outflow tract (OFT). Ts1Rhr hearts were no different to controls.
Significant difference to corresponding wild-type incidence (Fisher's exact test): §P < 0.05, *P < 0.0001, and #P = 0.034.