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. 2010 Sep 10;88(2):277–286. doi: 10.1093/cvr/cvq293

Figure 2.

Figure 2

Mitochondrial translocation of Cx-43 in preconditioned stem cells. (A) Western immunoblot showing purity of mitochondrial subfractionations in Sca-1+ cells using different concentrations of digitonin (ranging from 0.6 to 6 mg/mL) in the mitochondria lysis buffer. VDAC and Cox-IV were used as the mitochondrial outer and inner membrane markers, respectively. MT-outer, mitochondria outer membrane; MT-inner, mitochondria inner membrane. (B) Western immunoblot showing translocation of Cx-43 subsequent to preconditioning of Sca-1+ cells using 100 μM IGF-1 (the number of experiments performed, n = 3). BSA, bovine serum albumin.