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. Author manuscript; available in PMC: 2011 Sep 15.
Published in final edited form as: J Immunol. 2010 Aug 18;185(6):3190–3198. doi: 10.4049/jimmunol.0903670

Figure 6. FICZ but not Kynurenine enhances TH17 cell differentiation in vitro.

Figure 6

Naïve CD4+ CD25− T cells were isolated from B6 WT spleens and cultured in the presence of immunomagnetic microbeads coated with anti-CD3/ anti-CD28 antibodies, TGF-β (4 ng/ml) and IL-6 (20ng/ml) for 5 days. FICZ (200 nM) or kynurenine (50 µM) were added at the start of culture. Cells were then stimulated for 6 hours in the presence of PMA, Ionomycin and Brefeldin A at which time they were harvested and surfaced stained for CD4. This was followed by intracellular staining for IL-17. Numbers are the percent of live CD4 T cells expressing IL-17. Solid thick line is experimental histogram and shaded histogram is control (anti-CD3/CD28, TGF-β and IL-6 alone). Number above gate is the percent IL-17 positive of experimental histogram. Percent IL-17 positive of control histogram is 15.4. This figure is representative of 2 independent experiments.