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. 2010 Nov;51(11):3103–3116. doi: 10.1194/jlr.M005041

Fig. 8.

Fig. 8.

L-FABP expression and addition of glucose both increase nuclear BODIPY C-16 localization. Mock-transfected control (A, C, E, G, and I) and L-FABP overexpressing (B, D, F, H, and J) L-cells were incubated with the nonmetabolizable fluorescent fatty acid BODIPY C-16 (green pixels) and the nuclear dye SYTO59 (red pixels) in the presence of 0 mM (A and B), 0.6 mM (C and D), 6 mM (E and F), 20 mM (G and H), and 30 mM (I and J) glucose and imaged by LSCM. Panels numbered 1 show the pixels of BODIPY C-16 (green) and SYTO59 (red), and panels numbered 2 show BODIPY C-16 pixels colocalizing with the STYO59 stain for each condition in yellow. L-FABP, liver fatty acid binding protein.