TABLE 3.
Binding affinity, energy transfer efficiency, and molecular distance between Cy3-labeled PPARα and Cy-5-labeled L-FABP proteins in the presence of glucose metabolites determined by FRET as a function of donor quenching
| Metabolite | Kd (nM) | E (%) | r (Å) |
|---|---|---|---|
| None | 31.3 ± 5.7 | 53.2 ± 4.5 | 49.0 ± 1.5 |
| 6 mM Glucose | 26.6 ± 4.4 | 59.6 ± 7.8 | 46.7 ± 2.7 |
| 20 mM Glucose | 12.5 ± 1.7 | 26.0 ± 1.2 | 59.6 ± 0.7 |
| 30 mM Glucose | 8.6 ± 1.2 | 27.7 ± 2.1 | 59.3 ± 1.1 |
| G-1-P | 47.9 ± 6.4 | 29.5 ± 3.0 | 58.0 ± 1.5 |
| G-6-P | 11.3 ± 1.9 | 25.0 ± 3.7 | 60.4 ± 1.9 |
FRET, fluorescence resonance energy transfer; G-1-P, glucose-1-phosphate; G-6-P, glucose-6-phosphate; L-FABP, liver fatty acid binding protein; PPAR, peroxisome proliferator-activated receptor.