TABLE 2.

LC-FA composition from the whole retinas collected from young, middle, and old age and age-matched AMD human donors

				Mole % of Total FAsd
Peak no.a	RT (min)b	FAsc	Common Name	Young Age Group	Middle Age Group	Old Age Group	Age-Matched AMD Group
1	23.51	14:0	Tetradecanoic acid	0.50 ± 0.06	0.60 ± 0.06	0.61 ± 0.05	0.81 ± 0.09
2	26.42	15:0	Pentadecanoic acid	0.15 ± 0.05	0.12 ± 0.02	0.11 ± 0.01	0.21 ± 0.04
3	29.06	16:1n-9	7-Hexadecenoic acid	0.91 ± 0.14	0.66 ± 0.09	0.62 ± 0.04	0.76 ± 0.09
4	29.24	16:1n-7	9-Hexadecenoic acid	0.48 ± 0.03	0.49 ± 0.05	0.36 ± 0.03	0.50 ± 0.07
5	30.39	16:0	PA	15.98 ± 1.18	16.37 ± 1.37	16.62 ± 0.79	19.64 ± 1.79
6	34.45	17:0	Heptadecanoic acid	0.12 ± 0.04	0.08 ± 0.02	0.07 ± 0.02	0.14 ± 0.02
7	36.61	18:3n-3	α-Linolenic acid	0.39 ± 0.07	0.24 ± 0.06	0.24 ± 0.05	0.30 ± 0.04
8	37.38	18:2n-6	LA	1.63 ± 0.13	2.12 ± 0.15	1.93 ± 0.20	2.35 ± 0.24
9	37.85	18:1n-9	OA	14.88 ± 0.46	16.04 ± 1.56	14.53 ± 1.11	17.14 ± 1.38
10	38.02	18:1n-7	11-Octadecenoic acid	2.79 ± 0.17	2.83 ± 0.12	2.57 ± 0.25	3.08 ± 0.23
11	39.18	18:0	Octadecanoic acid/SA	14.80 ± 1.15	13.50 ± 0.46	13.91 ± 0.53	15.45 ± 0.98
12	44.47	20:4n-6	AA	13.43 ± 0.34	14.26 ± 0.64	14.04 ± 0.47	13.04 ± 0.36
13	44.64	20:5n-3	5,8,11,14,17-EPA	0.27 ± 0.03	0.28 ± 0.03	0.24 ± 0.04	0.35 ± 0.05
14	45.18	20:3n-6	8,11,14- Eicosatrienoic acid	2.38 ± 0.28	2.63 ± 0.14	2.29 ± 0.27	2.57 ± 0.26
15	45.44	20:2n-7	8,11-Eicosadienoic acid	0.09 ± 0.03	0.13 ± 0.05	0.10 ± 0.03	0.18 ± 0.02
16	45.99	20:2n-9	11,14-Eicosadienoic acid	0.09 ± 0.02	0.09 ± 0.02	0.14 ± 0.02	0.16 ± 0.04
17	46.23	20:1n-9	9-Eicosenoic acid	0.24 ± 0.04	0.29 ± 0.04	0.37 ± 0.04	0.55 ± 0.14
18	46.51	20:1n-7	11-Eicosenoic acid	0.17 ± 0.06	0.10 ± 0.05	0.03 ± 0.00	0.04 ± 0.01
19	47.42	20:0	Eicosanoic acid	0.65 ± 0.03	0.66 ± 0.01	0.82 ± 0.09	1.11 ± 0.15
20	51.90	22:5n-6	4,7,10,13,16-Docosapentaenoic acid	1.42 ± 0.33	1.03 ± 0.28	1.41 ± 0.31	1.02 ± 0.17
21	52.30	22:6n-3	4,7,10,13,16,19-DHA	23.74 ± 1.48	21.75 ± 2.78	23.01 ± 1.65	16.49 ± 0.62e*
22	52.50	22:4n-6	7,10,13,16-Docosatetraenoic acid (adrenic acid)	2.21 ± 0.40	2.53 ± 0.38	2.82 ± 0.37	1.61 ± 0.29e*
23	52.76	22:5n-3	7,10,13,16,19-Docosapentaenoic acid	1.52 ± 0.29	1.94 ± 0.36	1.77 ± 0.34	1.24 ± 0.25
24	55.39	22:0	Behenic acid	0.31 ± 0.02	0.40 0.03	0.41 ± 0.04	0.41 ± 0.03
25	61.83	24:1n-9	15-Tetracosenoic acid	0.66 ± 0.15	0.56 ± 0.10	0.71 ± 0.13	0.60 ± 0.15
26	62.75	24:0	Tetracosanoic acid	0.20 ± 0.03	0.31 ± 0.03	0.28 ± 0.04	0.25 ± 0.04
Ratio of AA/DHA				0.58 ± 0.05	0.76 ± 0.17	0.63 ± 0.06	0.79 ± 0.02e*
Ratio of n-6/n-3 LC-PUFAs				0.83 ± 0.05	1.05 ± 0.20	0.91 ± 0.06	1.13 ± 0.04e*

^aPeak no. was shown in chromatograms of Fig. 1.

^bRetention time.

^cNumber of carbon atoms: number of double bonds, the position of first double bound.

^dMean ± SEM; young age group: n = 5, middle age group: n = 6, old age group: n = 5, age-matched AMD group: n = 8.

^e* Significant differences (P < 0.05) between old age and age-matched AMD group.