TABLE 3.

LC-FA composition from RPE/choroid collected from young, middle, and old age and age-matched AMD human donors

				Mole % of Total FAsd
Peak no.a	RT (min)b	FAsc	Common Name	Young Age Group	Middle Age Group	Old Age Group	Age-Matched AMD Group
1	23.51	14:0	Tetradecanoic acid	0.51 ± 0.07	0.60 ± 0.07	0.52 ± 0.06	0.75 ± 0.10
2	26.42	15:0	Pentadecanoic acid	0.15 ± 0.00	0.12 ± 0.01	0.13 ± 0.01	0.18 ± 0.03
3	29.06	16:1n-9	7-Hexadecenoic acid	0.41 ± 0.03	0.48 ± 0.05	0.48 ± 0.04	0.68 ± 0.14
4	29.24	16:1n-7	9-Hexadecenoic acid	0.70 ± 0.08	1.03 ± 0.19	0.96 ± 0.10	1.20 ± 0.14
5	30.39	16:0	PA	18.41 ± 1.00	17.77 ± 1.43	16.49 ± 0.94	19.20 ± 0.81
6	34.45	17:0	Heptadecanoic acid	0.17 ± 0.05	0.15 ± 0.03	0.11 ± 0.03	0.21 ± 0.03
7	36.61	18:3n-3	α-Linolenic acid	0.20 ± 0.05	0.22 ± 0.05	0.30 ± 0.04	0.34 ± 0.06
8	37.38	18:2n-6	LA	10.33 ± 0.69	9.46 ± 0.02	13.80 ± 0.61f*	16.31 ± 2.25
9	37.85	18:1n-9	OA	18.15 ± 0.44	18.94 ± 1.31	19.11 ± 1.31	20.81 ± 1.51
10	38.02	18:1n-7	11-Octadecenoic acid	1.92 ± 0.13	2.39 ± 0.30	2.07 ± 0.16	2.45 ± 0.17
11	39.18	18:0	Octadecanoic acid/SA	10.92 ± 0.51	9.80 ± 0.38	9.24 ± 0.26	9.20 ± 0.27
12	44.47	20:4n-6	AA	20.89 ± 2.87	17.61 ± 1.75	19.14 ± 0.30	17.19 ± 1.10
13	44.64	20:5n-3	5,8,11,14,17-EPA	0.28 ± 0.03	0.34 ± 0.05	0.38 ± 0.04	0.49 ± 0.09
14	45.18	20:3n-6	8,11,14- Eicosatrienoic acid	2.70 ± 0.26	2.64 ± 0.25	3.08 ± 0.51	2.33 ± 0.19
15	45.44	20:2n-7	8,11-Eicosadienoic acid	0.14 ± 0.06	0.16 ± 0.06	0.11 ± 0.02	0.21 ± 0.04
16	45.99	20:2n-9	11,14- Eicosadienoic acid	0.13 ± 0.05	0.13 ± 0.04	0.19 ± 0.05	0.11 ± 0.02
17	46.23	20:1n-9	9-Eicosenoic acid	0.13 ± 0.04	0.20 ± 0.02	0.16 ± 0.03	0.20 ± 0.01
18	46.51	20:1n-7	11-Eicosenoic acid	0.03 ± 0.01	0.03 ± 0.00	0.03 ± 0.01	0.02 ± 0.00
19	47.42	20:0	Eicosanoic acid	0.71 ± 0.02	0.57 ± 0.05	0.62 ± 0.07	0.62 ± 0.04
20	51.90	22:5n-6	4,7,10,13,16-Docosapentaenoic acid	0.96 ± 0.15	0.97 ± 0.27	0.93 ± 0.19	0.49 ± 0.07
21	52.30	22:6n-3	4,7,10,13,16,19-DHA	4.13 ± 0.44	9.22 ± 1.95e*	4.94 ± 0.18f*	2.72 ± 0.15g*
22	52.50	22:4n-6	7,10,13,16-Docosatetraenoic acid (adrenic acid)	3.67 ± 0.45	3.10 ± 0.53	3.22 ± 0.34	1.43 ± 0.11g*
23	52.76	22:5n-3	7,10,13,16,19-Docosapentaenoic acid	2.12 ± 0.48	2.27 ± 0.47	2.09 ± 0.26	1.31 ± 0.14
24	55.39	22:0	Behenic acid	0.76 ± 0.06	0.61 ± 0.06	0.64 ± 0.06	0.61 ± 0.04
25	61.83	24:1n-9	15-Tetracosenoic acid	0.48 ± 0.15	0.51 ± 0.19	0.49 ± 0.10	0.42 ± 0.03
26	62.75	24:0	Tetracosanoic acid	0.97 ± 0.14	0.70 ± 0.09	0.74 ± 0.06	0.54 ± 0.08
Ratio of AA/DHA				5.24 ± 0.49	2.42 ± 0.64e*	3.90 ± 0.19f*	6.41 ± 0.41g*
Ratio of n-6/n-3 LC-PUFAs				5.84 ± 0.42	3.23 ± 0.65e*	5.22 ± 0.22f*	7.97 ± 0.51g*

^aPeak no. was shown in chromatograms of Fig. 1.

^bRetention time.

^cNumber of carbon atoms: number of double bonds, the position of first double bound.

^dMean ± SEM; young age group: n = 5, middle age group: n = 6, old age group: n = 5, age-matched AMD group: n = 8.

^e* Significant differences (P < 0.05) between young and middle age group.

^f* Significant differences (P < 0.05) between middle and old age group.

^g* Significant differences (P < 0.05) between old and age-matched AMD age group.