TABLE 4.
VLC-PUFA composition from whole retinas collected from young, middle, and old age and age-matched AMD human donors
Peak Area Value Normalized with IS and Sample Weightd |
||||||
---|---|---|---|---|---|---|
Peak no.a | RT (min)b | FAsc | Young Age Group | Middle Age Group | Old Age Group | Age-Matched AMD Group |
1 | 23.27 | 24:5n-6 | 185.24 ± 49.86 | 193.23 ± 41.19 | 147.60 ± 27.43 | 90.45 ± 18.65 |
2 | 23.43 | 24:6n-3 | 677.13 ± 237.78 | 668.48 ± 93.48 | 463.55 ± 55.82 | 151.65 ± 35.28g* |
3 | 23.58 | 24:4n-6 | 1199.82 ± 315.71 | 1188.55 ± 142.75 | 1153.45 ± 210.28 | 684.20 ± 126.50 |
4 | 23.74 | 24:5n-3 | 1315.65 ± 537.16 | 1442.42 ± 246.56 | 1124.25 ± 199.82 | 468.76 ± 111.35 |
5 | 27.55 | 26:5n-6 | 6.64 ± 2.46 | 10.74 ± 2.50 | 4.07 ± 0.99f* | 3.49 ± 0.92 |
6 | 27.72 | 26:6n-3 | 25.77 ± 8.76 | 27.74 ± 4.07 | 23.73 ± 5.22 | 10.45 ± 1.78 |
7 | 28.00 | 26:4n-6 | 60.61 ± 14.54 | 74.85 ± 4.88 | 58.90 ± 17.90 | 23.34 ± 4.20g* |
8 | 28.22 | 26:5n-3 | 57.07 ± 15.61 | 65.93 ± 6.25 | 46.70 ± 11.28 | 22.68 ± 4.36 |
9 | 33.61 | 28:6n-3 | 2.15 ± 0.61 | 2.09 ± 0.52 | 1.04 ± 0.45 | 0.13 ± 0.07 |
10 | 33.95 | 28:4n-6 | 2.54 ± 0.68 | 5.47 ± 0.72e* | 2.63 ± 0.88f* | 0.44 ± 0.24g* |
11 | 34.42 | 28:5n-3 | 8.71 ± 2.16 | 10.25 ± 1.23 | 7.51 ± 3.25 | 0.93 ± 0.50g* |
12 | 40.82 | 30:6n-3 | 2.62 ± 0.95 | 4.94 ± 0.93e* | 2.07 ± 0.73f* | 0.66 ± 0.26 |
13 | 41.42 | 30:4n-6 | 3.61 ± 1.08 | 6.84 ± 1.46 | 4.29 ± 1.79 | 0.90 ± 0.34g* |
14 | 41.82 | 30:5n-3 | 5.57 ± 1.82 | 12.98 ± 2.16e* | 4.93 ± 1.00f* | 2.20 ± 0.70 |
15 | 48.84 | 32:5n-6 | 8.45 ± 2.76 | 14.30 ± 2.27 | 7.52 ± 2.40f* | 1.51 ± 0.38g* |
16 | 49.32 | 32:6n-3 | 31.36 ± 9.66 | 47.84 ± 5.48 | 25.11 ± 5.78f* | 3.97 ± 0.97g* |
17 | 50.00 | 32:4n-6 | 40.12 ± 11.35 | 80.71 ± 15.27e* | 33.26 ± 8.27f* | 9.20 ± 3.21 |
18 | 50.46 | 32:5n-3 | 20.20 ± 5.97 | 50.74 ± 9.02e* | 21.53 ± 6.22f* | 3.93 ± 1.35g* |
19 | 58.49 | 34:6n-3 | 20.61 ± 8.43 | 25.94 ± 10.77 | 15.31 ± 3.80 | 1.71 ± 0.42 |
20 | 59.21 | 34:4n-6 | 25.37 ± 8.45 | 36.43 ± 5.76 | 16.50 ± 4.49f* | 2.41 ± 0.77g* |
21 | 59.72 | 34:5n-3 | 57.05 ± 28.08 | 105.06 ± 15.67 | 38.49 ± 16.01f* | 7.35 ± 2.19 |
Ratio of n-6/n-3 VLC-PUFAs | 0.83 ± 0.11 | 0.70 ± 0.11 | 0.83 ± 0.09 | 1.24 ± 0.10g* | ||
Sum of all VLC-PUFAs | 3757.77 ± 1205.26 | 4076.73 ± 458.17 | 3202.96 ± 535.34 | 1354.99 ± 268.34g* |
Peak no. was shown in chromatograms of Fig. 2.
Retention time.
Number of carbon atoms: number of double bonds, the position of first double bound.
Mean ± SEM; young age group: n = 5, middle age group: n = 6, old age group: n = 5, age-matched AMD group: n = 8.
* Significant differences (P < 0.05) between young and middle age group.
* Significant differences (P < 0.05) between middle and old age group.
* Significant differences (P < 0.05) between old and age-matched AMD age group.