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. 2010 Nov;51(11):3217–3229. doi: 10.1194/jlr.M007518

TABLE 5.

VLC-PUFAs composition from pooled RPE/choroid collected from young, middle and old age and age-matched AMD human donors

Peak Area Value Normalized with IS and Sample Weightd
Peak no.a RT (min)b FAsc Young Age Group Middle Age Group Old Age Group Age-Matched AMD Group
1 23.27 24:5n-6 78.04 97.41 83.09 62.80
2 23.43 24:6n-3 147.30 376.30 259.11 66.48
3 23.58 24:4n-6 321.66 522.54 365.28 148.77
4 23.74 24:5n-3 161.86 465.52 266.57 96.15
5 27.55 26:5n-6 2.44 3.88 3.01 1.78
6 27.72 26:6n-3 6.63 12.73 6.23 1.68
7 28.00 26:4n-6 16.08 18.22 17.68 5.59
8 28.22 26:5n-3 9.11 21.72 14.83 5.02
9 33.61 28:6n-3 0.00 0.00 0.00 0.00
10 33.95 28:4n-6 0.00 0.00 0.00 0.00
11 34.42 28:5n-3 0.00 0.00 0.00 0.00
12 40.82 30:6n-3 0.00 0.37 0.00 0.00
13 41.42 30:4n-6 0.00 0.50 0.00 0.00
14 41.82 30:5n-3 0.00 0.70 0.00 0.00
15 48.84 32:5n-6 0.13 1.62 0.48 0.00
16 49.32 32:6n-3 0.78 8.12 2.23 0.00
17 50.00 32:4n-6 0.80 8.89 2.69 0.00
18 50.46 32:5n-3 0.54 6.52 1.94 0.00
19 58.49 34:6n-3 0.00 5.03 0.60 0.00
20 59.21 34:4n-6 0.00 4.58 0.46 0.00
21 59.72 34:5n-3 0.00 11.14 1.42 0.00
Ratio of n-6/n-3 VLC-PUFAs 1.28 0.72 0.85 1.29
Sum of all VLC-PUFAs 745.37 1565.79 1025.62 388.27
a

Peak no. was shown in chromatograms of Fig. 2.

b

Retention time.

c

Number of carbon atoms: number of double bonds: position of double bonds, the position of first double bound.

d

Average = peak area in pooled sample/n; young age group: n = 5, middle age group: n = 6, old age group: n = 5, age-matched AMD group: n = 8. No statistics are provided due to only one pooled sample in each group.