TABLE 1.

Correlation between the HDL, apo A-I, and HDL subfractions vs. fractional efflux of cholesterol mass or label from J774 cells

HDL Components/Subfractions	FC-Enriched		FC- and CE-Enriched
	% mass efflux	% label efflux	% mass efflux	% label efflux
HDL	0.2	0.4	0.3	NS
Apo A-I	0.3	0.3	0.4	0.2
pre β−1	NS	0.3	0.4	0.2
α-1 HDL	0.2	NS	0.2	NS
α-2 HDL	0.2	NS	0.5	NS
α-3 HDL	NS	0.4	NS	0.4
α-4 HDL	NS	0.2	NS	0.2
pre α−1 HDL	0.2	NS	NS	NS

J774 cells were enriched with FC or FC and CE by exposure to acLDL with or without the presence of ACAT inhibitor respectively. The cell cholesterol mass at T0 (before the efflux phase) was 53.1 ± 1.9 µg total cholesterol/mg cell protein for FC-enriched cells, and 89.9 ± 4.2 µg total cholesterol/mg cell protein for FC- and CE-enriched cells. The experiment has been repeated twice in triplicate and the value used for correlation is the average of six values. Pearson correlations (r²) were assessed between the HDL, apo A-I, and HDL subfractions and the % radiolabeled cholesterol efflux or % cholesterol mass efflux. The r² of the statistically significant correlations are presented in the table. The statistical significance of the correlations were assessed at P ≤ 0.05. NS = nonsignificant.