TABLE 2.

Change in total cellular cholesterol mass in J774 cells

	Total Cholesterol Change(µg/mg cellular protein/8 h)
		Cholesterol-Enriched
	Cholesterol-Normal	FC-Enriched	FC- and CE-Enriched
3.5% Apo-B-depleted serum	+1.4 ± 0.3a	−16.7 ± 2.1a	−21.6 ± 3.2a
HDL3 (50 µg/ml)	+1.5 ± 0.6a	−12.7 ± 5.5a	−22.1 ± 4.3a
Apo A-I (25 µg/ml)	+0.6 ± 0.4	−6.6 ± 2.3a	−19.4 ± 6.8a

J774 cells were plated in growth medium for 24 h and labeled with medium containing 1% FBS and 2 µCi/ml ± 25 µg/ml acLDL for 24 h. The cells were equilibrated for 18 h in 0.2% BSA and the efflux was initiated by incubating the cells with 3.5% pooled apo-B-depleted human serum, HDL₃ (50 µg/ml), and apo A-I (25 µg/ml). ACAT inhibitor was added in the medium for cholesterol-normal and FC-enriched cells. The cell cholesterol mass at T0 (before the efflux phase) was 6.4 ± 0.3 µg total cholesterol/mg cell protein for cholesterol-normal cells, 53.1 ± 1.9 µg total cholesterol/mg cell protein for FC-enriched cells, and 89.9 ± 4.2 µg total cholesterol/mg cell protein for FC- and CE-enriched cells. Values are presented as the difference in cholesterol mass between the treatment at the end of efflux period and their corresponding T0 values. Plus sign indicates increase in total cellular cholesterol content, whereas minus sign indicates decrease in total cellular cholesterol content. Each value represents the average of triplicate. The data are given as mean ± SD.

^aStatistically significant from their corresponding T0 cholesterol mass content.