| DH10B |
E. coli; F− mcrA Δ(mrr-hsdRMS-mcrBC) phi80lacZΔM15 lacX74 recA1 endA1 araΔ139 Δ(ara leu)7697 galU galK lambda− rps (Strr) nupG
|
cloning strain |
LB |
Gibco BRL |
| S17-1 |
E. coli; recA thi pro hsdR [res– mod+][RP4::2-Tc::Mu-Km::Tn7] lambda-pir phage lysogen |
plasmid mobilization in B. melitensis
|
LB |
[41]
|
| BF264-15 Dau |
S. cerevisiae (MATalpha ade1 his2 leu2-3, 112 trp1-1a ura3) |
yeast strain used in this study for transformation with plasmids harboring LEU2 or URA3
|
Sc [42]
|
[43]; kindly provided by M. Grant, Wye College, UK |
| QX95001 |
BF264-15 Dau + pYEp51-bax
[44]
|
bax controlled by pGAL10 (pGAL10-bax) |
Sc-Leu |
[13]
|
| GL0001 |
QX95001 + pYES-DEST52-bclXL
|
pGAL10-bax + pGAL1-bclXL-V5-His6 |
Sc-Leu-Ura |
This study |
| GL0002 |
BF264-15 Dau + pYES-DEST52-BMEI1305 |
pGAL1-omp2b-V5-His6 |
Sc-Ura |
This study |
| GL0003 |
BF264-15 Dau + pYES-DEST52-BMEI0660 |
pGAL1-fecE-V5-His6 |
Sc-Ura |
This study |
| GL0004 |
QX95001 + pYES-DEST52-BMEI1305 |
pGAL10-bax + pGAL1-omp2b-V5-His6 |
Sc-Leu-Ura |
This study |
| GL0005 |
QX95001 + pYES-DEST52-BMEI0660 |
pGAL10-bax + pGAL1-fecE-V5-His6 |
Sc-Leu-Ura |
This study |
| GL0007 |
QX95001 + pYES-DEST52-BMEI1306 |
pGAL10-bax + pGAL1-omp2a-V5-His6 |
Sc-Leu-Ura |
This study |
| GL0008 |
QX95001 + pYES-DEST52-BMEI1249 |
pGAL10-bax + pGAL1-omp25-V5-His6 |
Sc-Leu-Ura |
This study |
| GL0010 |
BF264-15 Dau + pYES-DEST52-t-omp2b
|
pGAL1-t-omp2b-V5-His6 |
Sc-Ura |
This study |
| GL0011 |
QX95001 + pYES-DEST52-t-omp2b
|
pGAL10-bax + pGAL1-t-omp2b-V5-His6 |
Sc-Leu-Ura |
This study |
| GL0012 |
BF264-15 Dau + pYES-DEST52- short-omp2b
|
pGAL1-short-omp2b-V5-His6 |
Sc-Ura |
This study |
| GL0013 |
QX95001 + pYES-DEST52-short-omp2b
|
pGAL10-bax + pGAL1-short-omp2b-V5-His6 |
Sc-Leu-Ura |
This study |
| GL0014 |
BF264-15 Dau + pYES-DEST52-BMEI1306 |
pGAL1-omp2a-V5-His6 |
Sc-Ura |
This study |
| GL0015 |
BF264-15 Dau + pYES-DEST52-BMEI1249 |
pGAL1-omp25-V5-His6 |
Sc-Ura |
This study |
| GL0016 |
QX95001 + pYES-DEST52 |
pGAL10-bax + empty destination vector |
Sc-Leu-Ura |
This study |
| GL0017 |
QX95001 + pYES-DEST52-long-omp2a
|
pGAL10-bax + pGAL1-long-omp2a-V5-His6 |
Sc-Leu-Ura |
This study |
| GL0018 |
BF264-15 Dau + pYES-DEST52-long-omp2a
|
pGAL1-long-omp2a-V5-His6 |
Sc-Ura |
This study |
