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. 2010 Oct 11;5(10):e13305. doi: 10.1371/journal.pone.0013305

Figure 3. RA does not inhibit mature osteoclast function.

Figure 3

RAW264.7 cells were incubated with RANKL on bone slices or plastic for 5 days followed by incubation in 400 nM of RA for another 2 days (A–B). Human CD14+ cells were incubated on bone slices or plastic with M-CSF (M) and RANKL (RL) for 14 days or 10 days. The cells were then incubated in 400 nM of RA for another 2 days (D–E), or 12 days (D, F) on bone slices or on plastic for 2 days (C, D) or 8 days (G). The CTX measurements (E), secretion of TRAP activity (A, C, F, G) and the TRAP staining (B, D) were carried out as explained in Figure 1.