Figure 5. The effect of methyl-beta-cyclodextrin (MβCD).

A, Images representing overall activity after 200 frames (addition of all frames). Blue shade spots represent higher intensity, therefore an augment in vesicle residency over the recording. A 5 minutes treatment is enough to augment TRPM8-EGFP density at the plasma membrane. B, Variance analysis made on HEK293 cells stable expressing TRPM8 channels showing an increase in the average current correlated with a three fold increment in the number of active channels at the membrane (n = 4).