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. 2010 Oct 11;5(10):e13323. doi: 10.1371/journal.pone.0013323

Figure 2. Serine to alanine (A) or glutamic acid (E) single mutation or combined do not inactivate Sso1p or Sso2p in vivo.

Figure 2

A) The growth of serial 10-fold dilutions of sso1Δ sso2-1 cells expressing different mutant variants of Sso1p from a low copy plasmid at different temperatures. B) The mutant proteins generated are expressed at similar levels in S. cerevisiae cells. The sso1Δ sso2-1 cells (H2177) expressing different sso1 mutants were grown at designed temperatures and subjected to SDS-PAGE and Western blotting with anti-Sso1p or anti-Sso2p specific antibodies.