Table 1. Complementation capacity of sso and sec mutants.
Mutants | 24°C | 30°C | 34°C | 37°C | 38°C |
GAL1-SSO1 sso1Δ sso2Δ | |||||
Sso1 S59A | + | + | + | + | + |
Sso1 S59E | + | + | + | + | + |
Sso1 S59A S79A | + | + | + | + | + |
Sso1 S59E S79E | + | + | + | + | + |
Sso1 S23A S24A | + | + | + | + | + |
Sso1 S23E S24E | + | + | + | + | + |
Sso1 S23A S24A S59A | + | + | + | + | + |
Sso1 S23E S24E S59E | + | + | + | + | + |
Sso1 S23A S24A S59A S79A | + | + | + | + | + |
Sso1 S23E S24E S59E S79E | + | + | + | + | + |
Sso1 wt | + | + | + | + | + |
Sso2 T28A S31A S34A | + | + | + | + | + |
Sso2 T28E S31E S34E | + | + | + | + | + |
Sso2 wt | + | + | + | + | + |
Vector | − | − | − | − | − |
sso1 Δsso2-1 | |||||
Sso1 S59A | + | + | + | + | nd |
Sso1 S59E | + | + | + | + | nd |
Sso1 S59A S79A | + | + | + | + | nd |
Sso1 S59E S79E | + | + | + | + | nd |
Sso1 S23A S24A | + | + | + | + | nd |
Sso1 S23E S24E | + | + | + | + | nd |
Sso1 S23A S24 S59A | + | + | + | + | nd |
Sso1 S23E S24E S59E | + | + | + | + | nd |
Sso1 S23A S24A S59A S79A | + | + | + | + | nd |
Sso1 S23E S24E S59 S79E | + | + | + | + | nd |
Sso1 wt | + | + | + | + | nd |
Vector | + | − | − | − | nd |
sso1-1 sso2Δ | |||||
Sso2 T28A S31A S34A | + | + | + | + | + |
Sso2 T28E S31E S34E | + | + | + | + | + |
Sso2 wt | + | + | + | + | + |
Vector | + | + | + | + | − |
nd, not determined.