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. Author manuscript; available in PMC: 2010 Oct 11.
Published in final edited form as: Biochemistry. 2007 Feb 16;46(11):3566–3575. doi: 10.1021/bi062128k

Figure 2.

Figure 2

HsRad51 K133A (KA) mutant that repairs DNA breaks in vivo. Comet assays were performed to analyze Rad51-mediated repair of DNA double-strand breaks. (A) DNA from single HEK293 cells is visualized by EtBr staining following electrophoresis. Cells were exposed to IR as indicated and grown for 0.5 or 10 h. Results for cells expressing GFP-HsRAD51 transgenes in the presence (B) or absence (C) of Rad51-specific siRNA as indicated. At least 50 cells were analyzed for each time point, and all assays were performed in triplicate a minimum of four separate times. Student’s t-tests were performed to ensure the statistical significance of the data (see the text).