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Wild-type and mutant GFP-HsRad51 proteins interact with endogenous Rad51 and Xrcc3. Cells expressing the indicated fusion protein were lysed, precleared, and incubated with an anti-GFP monoclonal antibody. Following recovery of the antibody using protein G magnetic beads, Western blots were performed with antibodies specific for either HsRad51 or HsXrcc3. Controls marked GFP are from cells transfected with the pEGFP-C1 parental vector.
