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. Author manuscript; available in PMC: 2011 Nov 25.
Published in final edited form as: Virology. 2010 Sep 25;407(2):381–390. doi: 10.1016/j.virol.2010.08.036

Figure 2. Mobilization of SaPI1 by phage 53.

Figure 2

RN10822, a SaPI1-containing derivative of RN4220, was infected with either 80α or 53 as described in Materials and Methods. Standard minilysates were prepared at indicated times (minutes) after infection, separated on agarose, and probed for SaPI1 DNA by Southern blotting. The upper ‘bulk’ DNA band includes chromosomal DNA, replicating and linear phage-sized DNA, and replicating SaPI1 DNA; the lower band is SaPI1 linear monomers released from phage heads.