Skip to main content
. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Protein Expr Purif. 2010 Jun 10;74(2):242–247. doi: 10.1016/j.pep.2010.06.002

Figure 2.

Figure 2

Figure 2

Tris-glycine SDS-PAGE (10%) analysis of FMRP ISO1 purity (A) after the Ni-NTA affinity chromatography method developed herein and (B) prior to the optimization of the purification protocol. FMRP ISO1 is not expressed strongly enough in the presence of IPTG to be visibly distinguished from other protein bands in the cleared crude supernatant hence that sample is not shown. (A) Lane 1: EZ-Run protein marker (Fisher Scientific). Lanes 2-8: eluted pure FMRP ISO1 fractions 4-10, respectively. (B) Lane 1: EZ-Run protein marker. Lanes 2-5: eluted impure FMRP ISO1 fractions 5-8, respectively.