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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Protein Expr Purif. 2010 Jun 10;74(2):242–247. doi: 10.1016/j.pep.2010.06.002

Figure 5.

Figure 5

Fluorescence spectroscopy assay where FMRP ISO1 (black squares) is titrated into a fixed concentration of S3F-sh_8AP mRNA resulting in a Kd of 104 ± 11 nM and R2 value of 0.995. The Kd value was obtained by curve-fitting (red curve) the titration data points with equation 1. As a negative control, BSA (blue triangles) was similarly titrated and showed no significant binding to S3F-sh_8AP mRNA.