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(A) Electronic absorption spectra of the Fe(II) and Fe(II)-NO complexes for the two H-NOX proteins from L. pneumophila, showing the Soret and α/β region. Heme concentration was ~5 μm. (B) The predicted H-NOX operons from L. pneumophila. The gene coding for Hnox1 is found adjacent to a diguanylate cyclase gene. The gene coding for Hnox2 is found adjacent to a histidine kinase and single domain response regulator.
