Skip to main content
. Author manuscript; available in PMC: 2011 Dec 21.
Published in final edited form as: Mol Microbiol. 2010 Jun 21;77(4):930–942. doi: 10.1111/j.1365-2958.2010.07259.x

Figure 2.

Figure 2

Biofilm formation by LP02 and H-NOX mutants. A) Optical density was measured at 600 nm (OD 600) to quantify the growth of H-NOX mutant strains and LP02 in static culture in 96 well polystyrene microtiter plates. B) Biofilms were stained with crystal violet and resuspended in 95% ethanol before the absorbance at 600 nm (CV 600) was measured to quantify biofilm production for hnox1 mutant and LP02 in 96 well polystyrene microtiter plates. C) Relative biofilm formation (relative CV 600) at 5 days post-inoculation for all strains in 96 well polystyrene microtiter plates, and D) L. pneumophila was grown without shaking for 5 days in 5 mL polystyrene culture tubes, and then adherent cells (biofilms) were stained with crystal violet. *p-values<0.05 for comparison of Δhnox1 and LP02. **p-values<0.05 for comparison of Δhnox1 Δhnox2 and LP02.