Table 1.
Strains, plasmids and primers used in this study
| Strains and plasmids | Relevant characteristics | Source or reference |
|---|---|---|
| Bacterial strains | ||
| E. coli | ||
| DH5α λ pir | Mating strain | (Merriam et al., 1997, Ren et al., 2006) |
| 2174 | Helper strain | (Merriam et al., 1997, Ren et al., 2006) |
| BL21(DE3) pLysS | Expression strain | (Invitrogen) |
| L. pneumophila | ||
| LP02 | Wild-type L. pneumophila, thymidine auxotroph Smr | |
| Δhnox1 | LP02 Δhnox1 Smr | This work |
| Δhnox2 | LP02 Δhnox2 Smr | This work |
| Δhnox1 Δhnox2 | LP02 Δhnox1 Δhnox2 Smr | This work |
| Δhnox1 Δlpg1057 | LP02 Δhnox1 Δlpg1057 Smr | This work |
| LP02/pJB908 | LP02 pJB908 Smr Ampr | This work |
| Δhnox1/pJB908 | LP02 Δhnox1 pJB908Smr Ampr | This work |
| Δhnox2/pJB908 | LP02 Δhnox2 pJB908Smr Ampr | This work |
| Δhnox1 Δhnox2/pJB908 | LP02 Δhnox1 Δhnox2 pJB908 Smr Ampr | This work |
| LP02/pMMB206-GENT | LP02 pMMB206-GENT Smr Gentr | This work |
| LP02/pvca0956 | LP02 pvca0956 Smr Gentr | This work |
| LP02/plpg1057 | LP02 plpg1057 Smr Gentr | This work |
| Δhnox1/phnox1 | Δhnox1 phnox1 Smr Gentr | This work |
| Plasmids | ||
| pSR47S | sacB Kanr | (Merriam et al., 1997, Ren et al., 2006) |
| pSRhnox1 | psR47S::Δhnox1 sacB Kanr | This work |
| pSRhnox2 | psR47S::Δhnox2 sacB Kanr | This work |
| pSRlpg1057 | psR47S::Δlpg1057 sacB Kanr | This work |
| pJB908 | thymidylate synthetase Ampr | Gift from Prof. Ralph Isberg, Tufts Univ. |
| pMMB206-GENT | pTac promoter Gentr | (Hammer and Swanson, 1999) |
| plpg1057 | pMMB206-GENT::lpg1057 | This work |
| pvca0956 | pMMB206-GENT::vca0956 | This work |
| phnox1 | pMMB206-GENT::hnox1 | This work |
| pET20-b(+) | T7 promoter E. coli expression vector Ampr | Invitrogen |
| pEThnox1 | pET20-b(+)::Hnox1-His6 Ampr | This work |
| pETlpg1057 | pET20-b(+)::Lpg1057-His6 Ampr | This work |
| pAC1758 | pBAD33::Vca0956-His6, Cmr | Gift from Prof. Andrew Camilli, Tufts Univ. |