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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Bone. 2010 Aug 14;47(5):872–881. doi: 10.1016/j.bone.2010.08.007

Figure 2.

Figure 2

β-catenin nuclear translocation in mouse neonatal calvarial osteoblastic cultures. Panel A shows non-immune control immunostaining. Panel B shows the immunostaining of static cultures. Panel C and D show immunostaining after 2 hours of PFFSS at 2 or 16 dynes/cm2. Note the significant amount of cytoplasmic membrane associated positive staining in Panels B–D, whereas nuclear staining for β–catenin was only visible in Panel D. All cells were stained with an antibody that recognizes total β–catenin (red staining) and photographed with a 20X objective lens. Scale bar is 100 µm and is the same for all panels.