Microtubule-interacting agents induce surface EpCAM expression in Hey cells. (A) Hey cells were treated with 10X IC50 values of a variety of drugs (see Table 1) for 48 h. (B) Hey cells were treated with 10X IC50 values of Taxol, EpoB, Disco, VBL and TSA for 1 h, 24 h and 48 h, washed, fresh medium added, and incubation continued at 37°C for a total of 48 h. Cells were collected, immunostained with FITC-IgG or anti-HEA- (EpCAM)-FITC, followed by FACS analysis as described in Materials and Methods.