Table 1. Flourescent modification of MDA adducts.
Fluorescence on MDA or MAA modified albumin. Bovine serum albumin was modified with increasing concentrations of MDA alone or MDA with 1 mM AA (MAA), and assayed fluorescence at 398 nm and 460 nm. Data expressed are the ± S.E.M. of five separate experiments.
Adduct | nm/mg of MAA Flouresence | |
---|---|---|
MDA only | AA 1mM MDA increased | |
MDA 0.5 | 0.18 ± .06 | 0.03 ± .03 |
MDA 1.0 | 1.13 ± .52 | 23.06 ± 4.28 |
MDA 2.0 | 4.87 ± .78 | 51.69 ± 3.34 |
MDA 5.0 | 15.06 ± 2.76 | 141.86 ± 11.21 |
MDA 10.0 | 32.82 ± 7.63 | 289.99 ± 23.36 |
MDA 50.0 | 54.28 ± 9.60 | 504.90 ± 58.43 |
MDA 100.0 | 29.20 ± 3.36 | 714.64 ± 67.51 |