Table 2. Antibody Concentration to MDA adducts.

Relative antibody concentrations to MDA or MAA adduct using UNMC or Commercially available antibodies. Albumin (Alb), Alb modified with increasing concentrations of MDA, and MAA-Alb (2:1) were coated on ELISA plates and incubated with monoclonal or polyclonal antibodies to MDA/MAA adducts. Data expressed are the ± S.E.M. of five separate experiments.

	Monoclonal Antibodies		Polyclonal Antibodies
Concentration of MDA	Abcam MS × MDA	UNMC Ms × MAA	Calbiochem Rb × MDA	Biodesign Gt × MDA	UNMC Rb × MAA
MDA 0.5	2.30 × 105 ± 1.60 × 103	0	1.39 × 106 ± 3.13 × 105	2.75 × 105 ± 1.1 × 104	4.05 × 104 ± 1.66 × 103
MDA 1.0	2.97 × 105 ± 1.67 × 104	0	2.83 × 106 ± 3.28 × 105	6.25 × 105 ± 2.19 × 104	6.67 × 104 ± 5.72 × 103
MDA 2.5	8.53 × 105 ± 5.10 × 104	9.67 × 103 ± 1.69 × 102	4.46 × 106 ± 4.33 × 105	1.25 × 106 ± 4.11 × 105	2.47 × 105 ± 5.20 × 104
MDA 5.0	3.25 × 106 ± 2.99 × 105	9.99 × 104 ± 4.79 × 103	4.60 × 106 ± 9.49 × 105	2.15 × 106 ± 5.61 × 105	3.83 × 105 ± 2.68 × 104
MDA 10.0	3.16 × 106 ± 3.48 × 105	7.51 × 105 ± 2.76 × 104	6.35 × 106 ± 1.35 × 105	3.47 × 106 ± 8.41 × 105	5.83 × 105 ± 5.31 × 104
MDA 50.0	2.50 × 106 ± 5.10 × 105	1.54 × 106 ± 3.00 × 105	1.08 × 107 ± 2.23 × 105	5.07 × 106 ± 8.12 × 105	7.05 × 105 ± 5.40 × 104
MDA 100.0	2.75 × 106 ± 4.46 × 105	1.48 × 106 ± 3.02 × 105	8.13 × 106 ± 1.75 × 105	4.57 × 106 ± 6.10 × 105	5.80 × 105 ± 5.41 × 104
MAA 2:1	3.10 × 106 ± 1.95 × 105	3.10 × 106 ± 1.95 × 105	4.76 × 106 ± 9.84 × 105	3.52 × 106 ± 6.18 × 105	6.94 × 105 ± 3.09 × 104